Iranian Journal of Pediatrics

Published by: Kowsar

E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children

Ahmad Reza Rahnemoon 1 , * , Leila Koochakzadeh 2 , 3 , Shahla Ansari 4 , Anna Boyajyan 5 and Arsen Arakelyan 5
Authors Information
1 Allied Medical School, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IR Iran
2 Children’s Medical Center, Pediatrics Center of Excellence, Tehran, IR Iran
3 Department of Pediatrics, Tehran University of Medical Sciences, Tehran, IR Iran
4 Department of Pediatrics, Iran University of Medical Sciences, Tehran, IR Iran
5 Institute of Molecular Biology NAS RA, Yerevan, Armenia
Article information
  • JOURNAL DEMO NEOSCRIBER V3 single: June 2018, 28 (3); e5371
  • Published Online: May 29, 2018
  • Article Type: Research Article
  • Received: January 12, 2016
  • Revised: January 24, 2018
  • Accepted: January 27, 2018
  • DOI: 10.5812/ijp.5371

To Cite: Rahnemoon A R, Koochakzadeh L, Ansari S, Boyajyan A, Arakelyan A. et al. E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children, Iran J Pediatr. 2018 ;28(3):e5371. doi: 10.5812/ijp.5371.

Copyright © 2018, Copyright Holder Demo. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Pui CH, Crist WM. Biology and treatment of acute lymphoblastic leukemia. J Pediatr. 1994;124:491-503.
  • 2. Roberts WM, Rivera GK, Raimondi SC. Intensive chemotherapy for Philadelphia-chromosome-positive acute lymphoblastic leukemia. Lancet. 1994;343:331-2.
  • 3. Hantschel O. Targeting BCR-ABL and JAK2 in Ph+ ALL. Blood. 2015;125(9):1362-3. doi: 10.1182/blood-2014-12-617548. [PubMed: 25721043].
  • 4. Look AT. Oncogenic transcription factors in the human acute leukemias. Sci. 1997;78:1059-64.
  • 5. Cimino G, Elia L, Rapanotti MC. A prospective study of residual disease monitoring of the MLL/AF4 transcription patients with t(4;11) acute lymphoblastic leukemia. Blood. 2000;5:96-101.
  • 6. Pui CH, Chessells JM, Camitta B, Baruchel A, Biondi A, Boyett JM, et al. Clinical heterogeneity in childhood acute lymphoblastic leukemia with 11q23 rearrangements. Leukemia. 2003;17(4):700-6. doi: 10.1038/sj.leu.2402883. [PubMed: 12682627].
  • 7. Uckun FM, Sensel MG, Sather HN, Gaynon PS, Arthur DC, Lange BJ, et al. Clinical significance of translocation t(1;19) in childhood acute lymphoblastic leukemia in the context of contemporary therapies: a report from the Children's Cancer Group. J Clin Oncol. 1998;16(2):527-35. doi: 10.1200/JCO.1998.16.2.527. [PubMed: 9469337].
  • 8. Hutchison RE, Schexneider KI. Leukocytic disorders. In McPherson RA, Pincus MR. Henry's clinical diagnosis and management by laboratory methods. 23rd edition. China: Elsevier; 2017. p. 606-58.
  • 9. Catovsky D, Matutes E, Buccheri V, Shetty V, Hanslip J, Yoshida N, et al. A classification of acute leukaemia for the 1990s. Ann Hematol. 1991;62(1):16-21. [PubMed: 2031964].
  • 10. Campana D, Behm GB. Immunophenotyping of leukemia. J Immunol Method. 2000;243:59-75.
  • 11. Von Dongen JJ, Macintyre EA, Gabert JA. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BioMED-1 concerted action: investigation of minimal residual disease in acute leukemia. Leukemia. 1999;13:1901-28.
  • 12. Yeoh EJ, Ross ME, Shurtleff SA. Classification, subtype discovery, and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling. Cancer Cell. 2002;1:133-43.
  • 13. Armstrong SA, Staunton JE, Silverman LB, Pieters R, den Boer ML, Minden MD, et al. MLL translocations specify a distinct gene expression profile that distinguishes a unique leukemia. Nat Genet. 2002;30(1):41-7. doi: 10.1038/ng765. [PubMed: 11731795].
  • 14. Moos PJ, Raetz EA, Carlson MA, Szabo A, Smith FE, Willman C, et al. Identification of gene expression profiles that segregate patients with childhood leukemia. Clin Cancer Res. 2002;8(10):3118-30. [PubMed: 12374679].
  • 15. van der Burg M, Poulsen TS, Hunger SP, Beverloo HB, Smit EM, Vang-Nielsen K, et al. Split-signal FISH for detection of chromosome aberrations in acute lymphoblastic leukemia. Leukemia. 2004;18(5):895-908. doi: 10.1038/sj.leu.2403340. [PubMed: 15042105].
  • 16. Mullighan CG. The molecular genetic makeup of acute lymphoblastic leukemia. Hematology Am Soc Hematol Educ Program. 2012;2012:389-96. doi: 10.1182/asheducation-2012.1.389. [PubMed: 23233609].
  • 17. Kubicka M, Soszynska K, Mucha B, Rafinska B, Kolodziej B, Haus O, et al. Unusual profiles of pediatric acute lymphoblastic leukemia with MLL gene rearrangement. Leuk Lymphoma. 2007;48(10):2083-6. doi: 10.1080/10428190701606826. [PubMed: 17917979].
  • 18. Trka J, Zuna J, Haskovec C. Detection of BCR/ABL, MLL/AF4 and TEL/AML, hybrid genes and monitoring of minimal residual disease in pediatric patients with acute lymphoblastic leukemia. Cas Lek Cesk. 1991;138:12-7.
  • 19. Soszynska K, Mucha B, Debski R, Skonieczka K, Duszenko E, Koltan A, et al. The application of conventional cytogenetics, FISH, and RT-PCR to detect genetic changes in 70 children with ALL. Ann Hematol. 2008;87(12):991-1002. doi: 10.1007/s00277-008-0540-6. [PubMed: 18633615].
  • 20. Wu J, Zhang LP, Chen SH, Wang SQ. [Detection of fusion genes in childhood acute lymphoblastic leukemia by using oligonucleotide microarrays approach]. Zhonghua Er Ke Za Zhi. 2008;46(3):198-202. [PubMed: 19099709].
  • 21. Kager L, Lion T, Attarbaschi A, Koenig M, Strehl S, Haas OA, et al. Incidence and outcome of TCF3-PBX1-positive acute lymphoblastic leukemia in Austrian children. Haematologica. 2007;92(11):1561-4. doi: 10.3324/haematol.11239. [PubMed: 18024406].
  • 22. Kohlmann A, Schoch C, Schnittger S, Dugas M, Hiddemann W, Kern W, et al. Pediatric acute lymphoblastic leukemia (ALL) gene expression signatures classify an independent cohort of adult ALL patients. Leukemia. 2004;18(1):63-71. doi: 10.1038/sj.leu.2403167. [PubMed: 14603332].
  • 23. Sudhakar N, Rajalekshmy KR, Rajkumar T, Nancy KN. RT-PCR and real-time PCR analysis of E2A-PBX1, TEL-AML1, mBCR-ABL and MLL-AF4 fusion gene transcripts in de novo B-lineage acute lymphoblastic leukaemia patients in south India. J Genet. 2011;90(2):349-53. [PubMed: 21869488].
  • 24. Zuo YX, Zhang LP, Lu AD, Wang B, Liu GL. [Clinical characteristics of children with B cell type acute lymphoblastic leukemia carrying different fusion gene]. Zhongguo Dang Dai Er Ke Za Zhi. 2010;12(3):172-6. [PubMed: 20350423].
  • 25. Mesquita DR, Cordoba JC, Magalhaes IQ, Cordoba MS, Oliveira JR, Goncalves A, et al. Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazil's Federal District. Genet Mol Res. 2009;8(1):345-53. [PubMed: 19440970].
  • 26. Cetin Z, Yakut S, Karadogan I, Kupesiz A, Timuragaoglu A, Salim O, et al. Aberrations of chromosomes 9 and 22 in acute lymphoblastic leukemia cases detected by ES-fluorescence in situ hybridization. Genet Test Mol Biomarkers. 2012;16(5):318-23. doi: 10.1089/gtmb.2011.0186. [PubMed: 22360868].
  • 27. Qin YZ, Li JL, Zhu HH, Li LD, Chang Y, Le H, et al. [Detection of common fusion transcript levels in untreated leukemia patients by real-time quantitative RT-PCR technique]. Zhonghua Xue Ye Xue Za Zhi. 2007;28(7):433-7. [PubMed: 18072623].
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments