Iranian Journal of Pediatrics

Published by: Kowsar

Apoptotic Changes in the Early Diagnosis and Severity Determination of Neonatal Sepsis

Naglaa Fathy Barseem 1 , * and Mohamed Ahmed Helwa 2
Authors Information
1 Pediatric Department, Faculty of Medicine, Menoufia University, Egypt
2 Clinical Pathology Department, Faculty of Medicine, Menoufia University, Egypt
Article information
  • Iranian Journal of Pediatrics: February 2017, 27 (1); e7701
  • Published Online: August 31, 2016
  • Article Type: Research Article
  • Received: June 28, 2016
  • Revised: July 31, 2016
  • Accepted: August 23, 2016
  • DOI: 10.5812/ijp.7701

To Cite: Barseem N F, Helwa M A. Apoptotic Changes in the Early Diagnosis and Severity Determination of Neonatal Sepsis, Iran J Pediatr. 2017 ; 27(1):e7701. doi: 10.5812/ijp.7701.

Copyright © 2017, Growth & Development Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. van den Hoogen A, Gerards LJ, Verboon-Maciolek MA, Fleer A, Krediet TG. Long-term trends in the epidemiology of neonatal sepsis and antibiotic susceptibility of causative agents. Neonatology. 2010; 97(1): 22-8[DOI][PubMed]
  • 2. Chan KY, Lam HS, Cheung HM, Chan AK, Li K, Fok TF, et al. Rapid identification and differentiation of Gram-negative and Gram-positive bacterial bloodstream infections by quantitative polymerase chain reaction in preterm infants. Crit Care Med. 2009; 37(8): 2441-7[DOI][PubMed]
  • 3. Narasimha A, Harendra Kumar ML. Significance of Hematological Scoring System (HSS) in Early Diagnosis of Neonatal Sepsis. Indian J Hematol Blood Transfus. 2011; 27(1): 14-7[DOI][PubMed]
  • 4. Hawk M. C-reactive protein in neonatal sepsis. Neonatal Netw. 2008; 27(2): 117-20[DOI][PubMed]
  • 5. Serhan CN. Resolution phase of inflammation: novel endogenous anti-inflammatory and proresolving lipid mediators and pathways. Annu Rev Immunol. 2007; 25: 101-37[DOI][PubMed]
  • 6. Leitch AE, Duffin R, Haslett C, Rossi AG. Relevance of granulocyte apoptosis to resolution of inflammation at the respiratory mucosa. Mucosal Immunol. 2008; 1(5): 350-63[DOI][PubMed]
  • 7. Martin C, Burdon PC, Bridger G, Gutierrez-Ramos JC, Williams TJ, Rankin SM. Chemokines acting via CXCR2 and CXCR4 control the release of neutrophils from the bone marrow and their return following senescence. Immunity. 2003; 19(4): 583-93[PubMed]
  • 8. Serhan CN, Brain SD, Buckley CD, Gilroy DW, Haslett C, O'Neill LA, et al. Resolution of inflammation: state of the art, definitions and terms. FASEB J. 2007; 21(2): 325-32[DOI][PubMed]
  • 9. Sankar MJ, Agarwal R, Deorari AK, Paul VK. Sepsis in the newborn. Indian J Pediatr. 2008; 75(3): 261-6[PubMed]
  • 10. Tollner U. Early diagnosis of septicemia in the newborn. Clinical studies and sepsis score. Eur J Pediatr. 1982; 138(4): 331-7[PubMed]
  • 11. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-91[PubMed]
  • 12. Young Infants Clinical Signs Study G. Clinical signs that predict severe illness in children under age 2 months: a multicentre study. Lancet. 2008; 371(9607): 135-42[DOI][PubMed]
  • 13. Manucha V, Rusia U, Sikka M, Faridi MM, Madan N. Utility of haematological parameters and C-reactive protein in the detection of neonatal sepsis. J Paediatr Child Health. 2002; 38(5): 459-64[PubMed]
  • 14. Winn WC, Koneman EW. Koneman's color atlas and textbook of diagnostic microbiology. 2006;
  • 15. Hu CT, O'Shaughnessy KM. Glycerol-enhanced mini-polyacrylamide gel electrophoresis for the separation of differentially expressed DNA fragments in cDNA representational difference analysis. Electrophoresis. 2001; 22(6): 1063-8[DOI][PubMed]
  • 16. Kermorvant-Duchemin E, Laborie S, Rabilloud M, Lapillonne A, Claris O. Outcome and prognostic factors in neonates with septic shock. Pediatr Crit Care Med. 2008; 9(2): 186-91[DOI][PubMed]
  • 17. Stoll BJ. Infections of the Neonatal Infant. Nelson Textbook of Pediatrics. 2008; : 794-811
  • 18. Hornik CP, Fort P, Clark RH, Watt K, Benjamin DJ, Smith PB, et al. Early and late onset sepsis in very-low-birth-weight infants from a large group of neonatal intensive care units. Early Hum Dev. 2012; 88 Suppl 2-74[DOI][PubMed]
  • 19. Strunk T, Currie A, Richmond P, Simmer K, Burgner D. Innate immunity in human newborn infants: prematurity means more than immaturity. J Matern Fetal Neonatal Med. 2011; 24(1): 25-31[DOI][PubMed]
  • 20. El Kebir D, Filep JG. Role of neutrophil apoptosis in the resolution of inflammation. ScientificWorldJournal. 2010; 10: 1731-48[DOI][PubMed]
  • 21. Guida JD, Kunig AM, Leef KH, McKenzie SE, Paul DA. Platelet count and sepsis in very low birth weight neonates: is there an organism-specific response? Pediatrics. 2003; 111(6 Pt 1): 1411-5[PubMed]
  • 22. Priyadharshini NA, Parkash C. Assessment of DNA damage in term neonates with sepsis by comet assay. Curr Pediatr Res. 2014; 18(1)
  • 23. Carvalho NC, de Souza RL, Dal-Pizzol F, de Andrade VM. Comet assay in neonatal sepsis. Indian J Pediatr. 2010; 77(8): 875-7[DOI][PubMed]
  • 24. Delanghe JR, Speeckaert MM. Translational research and biomarkers in neonatal sepsis. Clin Chim Acta. 2015; 451: 46-64[DOI][PubMed]
  • 25. Meem M, Modak JK, Mortuza R, Morshed M, Islam MS, Saha SK. Biomarkers for diagnosis of neonatal infections: A systematic analysis of their potential as a point-of-care diagnostics. J Glob Health. 2011; 1(2): 201-9[PubMed]
  • 26. Garcia-Romo GS, Caielli S, Vega B, Connolly J, Allantaz F, Xu Z, et al. Netting neutrophils are major inducers of type I IFN production in pediatric systemic lupus erythematosus. Sci Transl Med. 2011; 3(73): 73ra20[DOI][PubMed]
  • 27. Yang KD, Chen MZ, Teng RJ, Yang MY, Liu HC, Chen RF, et al. A model to study antioxidant regulation of endotoxemia-modulated neonatal granulopoiesis and granulocyte apoptosis. Pediatr Res. 2000; 48(6): 829-34[DOI][PubMed]
  • 28. Scheel-Toellner D, Wang KQ, Webb PR, Wong SH, Craddock R, Assi LK, et al. Early events in spontaneous neutrophil apoptosis. Biochem Soc Trans. 2004; 32: 461-4[DOI][PubMed]
  • 29. Drifte G, Dunn-Siegrist I, Tissieres P, Pugin J. Innate immune functions of immature neutrophils in patients with sepsis and severe systemic inflammatory response syndrome. Crit Care Med. 2013; 41(3): 820-32[DOI][PubMed]
  • 30. Srinivasan L, Kirpalani H, Cotten CM. Elucidating the role of genomics in neonatal sepsis. Semin Perinatol. 2015; 39(8): 611-6[DOI][PubMed]
  • 31. Paunel-Gorgulu A, Kirichevska T, Logters T, Windolf J, Flohe S. Molecular mechanisms underlying delayed apoptosis in neutrophils from multiple trauma patients with and without sepsis. Mol Med. 2012; 18: 325-35[DOI][PubMed]
  • 32. Altrichter J, Sauer M, Kaftan K, Birken T, Gloger D, Gloger M, et al. Extracorporeal cell therapy of septic shock patients with donor granulocytes: a pilot study. Crit Care. 2011; 15(2)[DOI][PubMed]
  • 33. Payne CM, Glasser L, Tischler ME, Wyckoff D, Cromey D, Fiederlein R, et al. Programmed cell death of the normal human neutrophil: an in vitro model of senescence. Microsc Res Tech. 1994; 28(4): 327-44[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments